Journal: Journal of Molecular Medicine (Berlin, Germany)

Article Title: FATP1-mediated fatty acid uptake in renal tubular cells as a countermeasure for hypothermia

doi: 10.1007/s00109-025-02525-0

Figure Lengend Snippet: Effect of low temperature on cultured mouse proximal tubular cells. A Volcano plot representing the gene expression variation when mouse renal proximal tubular epithelial cells (MRPTEpiCs) were incubated at low temperature (28 °C, n = 5) compared with standard culture temperature (37 °C, n = 5). B Normalized enrichment score analyzed by gene set enrichment analysis (GSEA; Gene Ontology biological process). C Representative GSEA results. D Slc27a1 mRNA expression levels in MRPTEpiCs cultured with standard and low temperatures, RNA sequencing data (left) and qRT-PCR data (right) ( n = 5, respectively). E FATP1 protein expression levels in MRPTEpiCs analyzed by immunofluorescence staining. Scale bars: 10 μm. F Western blot analysis of FATP1 protein expression in MRPTEpiCs cultured at standard and low temperatures ( n = 5, respectively). G Analysis of lipid accumulation in MRPTEpiCs cultured at standard and low temperatures. BODYPI (green) and DAPI (blue). Scale bars: 10 μm. H Quantitative analysis of fluorescence intensity representing accumulated lipids in MRPTEpiCs cultured at standard and low temperatures ( n = 16 each). I Oxygen consumption rate (OCR) determined for MRPTEpiCs incubated under standard and low temperature conditions by Seahorse analysis. Oligomycin, FCCP (carbonyl cyanide-4-(trifluoromethoxy) phenylhydrazone) and rotenone/antimycin were added at the indicated time points ( n = 3, respectively). Data were analyzed by one-way ANOVA followed by the Mann–Whitney U test or Turkey-Kramer method. All data are represented as the mean ± standard error of the mean. * P < 0.05, ** P < 0.01, **** P < 0.0001. Cont: control group; Hyp: hypothermia group

Article Snippet: Mouse renal proximal tubular epithelial cells (MRPTEpiCs) were purchased from Innoprot (Bizkaia, Spain).

Techniques: Cell Culture, Gene Expression, Incubation, Expressing, RNA Sequencing, Quantitative RT-PCR, Immunofluorescence, Staining, Western Blot, Fluorescence, MANN-WHITNEY, Control

Journal: Journal of Molecular Medicine (Berlin, Germany)

Article Title: FATP1-mediated fatty acid uptake in renal tubular cells as a countermeasure for hypothermia

doi: 10.1007/s00109-025-02525-0

Figure Lengend Snippet: Effect of FATP1 suppression or renal injury on hypothermia. A Schematic depiction of the experimental design. RT: kept at room temperature; Hyp: kept at 4 °C; FATP1-in-1: mice treated with 20 mg/kg FATP1-in-1. B Changes in body temperature in FATP1-inhibited model mice ( n = 5 each). C FFA and TG levels in renal tissue extracts from the model mice ( n = 5 each). D Hematoxylin and eosin (H&E) staining and immunohistochemical staining of cleaved-PARP in the model mice. Scale bars: 50 μm. E The number of cleaved-PARP-positive cells in renal proximal tubular cells. The average of five fields of view at high magnification (× 400). F Cytotoxicity LDH assay and SRB assay in mouse renal proximal tubular epithelial cells (MRPTEpiCs) incubated under standard (37 °C) and low-temperature (28 °C) conditions with palmitic acid (PA) and FATP1-inhibitor ( n = 6). Data were analyzed by the Mann–Whitney U test or one-way ANOVA followed by the Turkey-Kramer method. All data are represented as the mean ± standard error of the mean. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. Cont: control group; Hyp: hypothermia group; FATP1-in-1 RT: FATP1 inhibitor room temperature group; FATP1-in-1 Hyp: FATP1 inhibitor hypothermia group

Article Snippet: Mouse renal proximal tubular epithelial cells (MRPTEpiCs) were purchased from Innoprot (Bizkaia, Spain).

Techniques: Staining, Immunohistochemical staining, Lactate Dehydrogenase Assay, Sulforhodamine B Assay, Incubation, MANN-WHITNEY, Control

Journal: Frontiers in Pharmacology

Article Title: Molecular Mechanisms of Curcumin Renoprotection in Experimental Acute Renal Injury

doi: 10.3389/fphar.2017.00912

Figure Lengend Snippet: Expression of APPL1 and phosphorylated Akt in an in vitro curcumin treated HR model. (A) Representative Western blots show APPL1 protein and phosphorylated Akt protein levels in renal tubular epithelial cells of each group. (B) Quantitative analysis of APPL1 protein levels in the kidneys. a P < 0.05 vs. Sham group; b P < 0.05 vs. HR group; c P < 0.05 vs. HR+Curcumin group. n = 4 in each group. (C) Quantitative analysis of phosphorylated Akt protein levels in the kidneys. a P < 0.05 vs. Sham group; b P < 0.05 vs. HR group; c P < 0.05 vs. HR+Curcumin group; d P < 0.05 vs. HR+Curcumin+APPL1 siRNA group. n = 4 in each group.

Article Snippet: Mouse renal tubular epithelial cells (EpiCM-a) (Sciencell, San Diego, California, US) were thawed in a 38°C water bath and then centrifuged at 1,000 rpm for 5 min.

Techniques: Expressing, In Vitro, Western Blot

Journal: Frontiers in Pharmacology

Article Title: Molecular Mechanisms of Curcumin Renoprotection in Experimental Acute Renal Injury

doi: 10.3389/fphar.2017.00912

Figure Lengend Snippet: The effect of curcumin on TUNEL positive renal tubular epithelial cells treated with HR. (A) TUNEL apoptosis assay in mouse renal tubular epithelial cells. Scale bar: 100 μm. (B) Quantitative analysis of the apoptosis ratio of renal tubular epithelial cells in each group. a P < 0.05 vs. Sham group; b P < 0.05 vs. HR group; c P < 0.05 vs. HR+Curcumin group; d P < 0.05 vs. HR+Curcumin+APPL1 siRNA group. n = 4 in each group.

Article Snippet: Mouse renal tubular epithelial cells (EpiCM-a) (Sciencell, San Diego, California, US) were thawed in a 38°C water bath and then centrifuged at 1,000 rpm for 5 min.

Techniques: TUNEL Assay, Apoptosis Assay

Journal: Frontiers in Pharmacology

Article Title: Molecular Mechanisms of Curcumin Renoprotection in Experimental Acute Renal Injury

doi: 10.3389/fphar.2017.00912

Figure Lengend Snippet: Flow cytometric analysis of the apoptosis rate of renal tubular epithelial cells in each group. (A) Flow cytometric analysis of the apoptosis rate of mouse renal tubular epithelial cells. (B) The apoptosis rate of renal tubular epithelial cells in each group in the early period. a P < 0.05 vs. Sham group; b P < 0.05 vs. HR group; c P < 0.05 vs. HR+Curcumin group; d P < 0.05 vs. HR+Curcumin+APPL1 siRNA group. n = 4 in each group. (C) The apoptosis rate of renal tubular epithelial cells in each group in the later period. a P < 0.05 vs. Sham group; b P < 0.05 vs. HR group; c P < 0.05 vs. HR+Curcumin group; d P < 0.05 vs. HR+Curcumin+APPL1 siRNA group. n = 4 in each group. (D) The total apoptosis rate of renal tubular epithelial cells in each group in the later period. a P < 0.05 vs. Sham group; b P < 0.05 vs. HR group; c P < 0.05 vs. HR+Curcumin group; d P < 0.05 vs. HR+Curcumin+APPL1 siRNA group. n = 4 in each group.

Article Snippet: Mouse renal tubular epithelial cells (EpiCM-a) (Sciencell, San Diego, California, US) were thawed in a 38°C water bath and then centrifuged at 1,000 rpm for 5 min.

Techniques:

Journal: Frontiers in Pharmacology

Article Title: Molecular Mechanisms of Curcumin Renoprotection in Experimental Acute Renal Injury

doi: 10.3389/fphar.2017.00912

Figure Lengend Snippet: The expression of Bax and C-Caspase-3 in renal tubular epithelial cells in each group. (A) Representative Western blots show Bax and C-Caspase-3 protein levels of renal tubular epithelial cells in each group. (B) Quantitative analysis of Bax protein levels in renal tubular epithelial cells. a P < 0.05 vs. Sham group; b P < 0.05 vs. HR group; c P < 0.05 vs. HR+Curcumin group; d P < 0.05 vs. HR+Curcumin+APPL1 siRNA group. n = 4 in each group. (C) Quantitative analysis of C-Caspase-3 protein levels in renal tubular epithelial cells. a P < 0.05 vs. Sham group; b P < 0.05 vs. HR group; c P < 0.05 vs. HR+Curcumin group; d P < 0.05 vs. HR+Curcumin+APPL1 siRNA group. n = 4 in each group.

Article Snippet: Mouse renal tubular epithelial cells (EpiCM-a) (Sciencell, San Diego, California, US) were thawed in a 38°C water bath and then centrifuged at 1,000 rpm for 5 min.

Techniques: Expressing, Western Blot